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Addgene

pET28a-mxEH
(Plasmid #104977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET 28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6265
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MXAN_1644
  • Alt name
    mxEH
  • Species
    Myxococcus xanthus
  • Insert Size (bp)
    957
  • GenBank ID
    ABF 91519.1
  • Entrez Gene
    MXAN_1644 (a.k.a. MXAN_1644)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6 histidine tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GCGCATATGGCTGACATCACGCATCGAAC
  • 3′ sequencing primer GCTAAGCTTTCAGGCCGGCAGCTTCTTCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-mxEH was a gift from Deokkun Oh (Addgene plasmid # 104977 ; http://n2t.net/addgene:104977 ; RRID:Addgene_104977)
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