pET28a-mxLOX2
(Plasmid #104976)

• Purpose: Biosynthesis of oxylipins by microbial enzymes
• Depositing Lab: Deokkun Oh
• Publication: Bacterial expression plasmids of microbial lipoxygenases originated from Myxococcus xanthus (unpublished)

Backbone

• Vector backbone: pET 28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7338
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MXAN_1744
• Alt name: mxLOX2
• Species: Myxococcus xanthus
• Insert Size (bp): 2028
• GenBank ID: ABF 88826.1
• Entrez Gene: MXAN_1744 (a.k.a. MXAN_1744)
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • 6 histidine tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GCCGCATATGACTGTCGAGTACAAAC
• 3′ sequencing primer: TACAAGCTTTCAGACGGTGATGCCG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry