pAAV2/2
(Plasmid #104963)

• Purpose: AAV packaging plasmid expressing Rep/Cap genes for production of serotype 2
• Depositing Lab: Melina Fan
• Publication: AAV Packaging (unpublished)

Backbone

• Vector backbone: pUC19 with additional elements
• Total vector size (bp): 7468
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rep2/Cap2
• Species: parvovirus
• Insert Size (bp): 4090
• Promoter: p5

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pAAV2/2 sequence.dna
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.