pSF1389
(Plasmid #104962)

• Purpose: Bacterial expression plasmid of bdSENP1 protease
• Depositing Lab: Dirk Görlich
• Publication: Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105. doi: 10.1016/j.chroma.2014.02.029. Epub 2014 Feb 19.

Backbone

• Vector backbone: pQE80 derivative
• Total vector size (bp): 4720
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SENP1
• Alt name: Sentrin/SUMO-specific protease SENP1
• Species: Brachypodium distachyon
• Insert Size (bp): 825
• Promoter: lacUV5
• Tags / Fusion Proteins:
  • His14 tag (N terminal on backbone)
  • Tev protease cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Acc65I (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: QE-fw
• 3′ sequencing primer: QE-rev

Resource Information

• Supplemental Documents:
  • pSF1389_His14-TEV-bdSENP1.gbk
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry