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pUASt-FMW-attB-AthHen1
(Plasmid #104958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASt-FMW
  • Backbone manufacturer
    Thermofisher
  • Backbone size w/o insert (bp) 11181
  • Total vector size (bp) 12671
  • Modifications to backbone
    Gibson cloning of AttB site into pUSt-FMW, followed by Gateway cloning of pENTR-D_AthHEN1-DmeCO (entry vector) and pUASt-FMW-attB (destination vector)
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hen1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2829
  • Entrez Gene
    HEN1 (a.k.a. AT4G20910, CORYMBOSA 2, CRM2, HUA ENHANCER 1, T13K14.70, T13K14_70)
  • Promoter Hsp70 promoter
  • Tag / Fusion Protein
    • FLAG Myc (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASt-FMW-attB-AthHen1 was a gift from Stefan Ameres (Addgene plasmid # 104958 ; http://n2t.net/addgene:104958 ; RRID:Addgene_104958)

  • For your References section:

    Cell-type specific sequencing of microRNAs from complex animal tissues. Alberti C, Manzenreither RA, Sowemimo I, Burkard TR, Wang J, Mahofsky K, Ameres SL, Cochella L. Nat Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610. 10.1038/nmeth.4610 PubMed 29481550
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