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Addgene

pCR8/GW:PtU6
(Plasmid #104895)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104895 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8/GW/TOPO
  • Backbone size w/o insert (bp) 2817

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Phaeodactylum U6 promoter
  • Species
    Phaeodactylum tricornutum
  • Insert Size (bp)
    280

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer CAGGAAACAGCTATGAC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    U6 promoter was cloned from gDNA based on Nymark M, Sharma AK, Sparstad T Bones, A, Winge P. A CRISPR/Cas9 system adapted for gene editing in marine algae.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For Golden Gated assembly.

Please visit https://www.biorxiv.org/content/early/2018/10/17/444109 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR8/GW:PtU6 was a gift from Thomas Mock (Addgene plasmid # 104895 ; http://n2t.net/addgene:104895 ; RRID:Addgene_104895)

  • For your References section:

    Biochemical Characterization of a Novel Redox-Regulated Metacaspase in a Marine Diatom. Graff van Creveld S, Ben-Dor S, Mizrachi A, Alcolombri U, Hopes A, Mock T, Rosenwasser S, Vardi A. Front Microbiol. 2021 Sep 8;12:688199. doi: 10.3389/fmicb.2021.688199. eCollection 2021. 10.3389/fmicb.2021.688199 PubMed 34566902
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