pActin5c-CD-dCas9m4-UDI-attB
(Plasmid
#104879)
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PurposeTo generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepActin5c-attB
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Backbone manufacturerPort and Bullock; PNAS 2014
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD-dCas9(m4)-UDI
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MUTATOR variant of dCas9; Fusion of cytidine deamine-deactivatedCas9(with 4 mutations)-Uracil DNA glycosylase; will cause point mutations of target C->T (or G->A) based on gRNA used.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pActin5c-CD-dCas9m4-UDI-attB was a gift from Christopher Potter (Addgene plasmid # 104879 ; http://n2t.net/addgene:104879 ; RRID:Addgene_104879)
