pHACK-GAL4>GAL80
(Plasmid
#104874)
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PurposeHACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104874 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHACK
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Backbone manufacturerPotter Lab
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2A-GAL80
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Alt namegRNA1 against GAL4
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Alt namegRNA2 against GAL4
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HACK vector for converting GAL4 lines to GAL80
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHACK-GAL4>GAL80 was a gift from Christopher Potter (Addgene plasmid # 104874 ; http://n2t.net/addgene:104874 ; RRID:Addgene_104874)