pMP71-HLA-B0702-His
(Plasmid #104850)

• Purpose: HLA-B0702 his tagged
• Depositing Lab: Sébastien Walchli
• Publication: Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 10.4049/immunohorizons.1800029.

Backbone

• Vector backbone: pMP71
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6750
• Modifications to backbone: converted to become Gateway compatible, see this paper: PMID: 22132171
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: major histocompatibility complex, class I, B
• Alt name: HLA-B0702
• Alt name: B*07:02:01:01
• Species: H. sapiens (human)
• Entrez Gene: HLA-B (a.k.a. AS, B-4901, HLAB)
• Promoter: LTR
• Tag / Fusion Protein:
  • his (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: none

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry