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pENTR-HLA-B0702-His
(Plasmid #104849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 3688
  • Vector type
    entry vector for Gateway system

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    major histocompatibility complex, class I, B
  • Alt name
    HLA-B0702
  • Alt name
    B*07:02:01:01
  • Species
    H. sapiens (human)
  • Entrez Gene
    HLA-B (a.k.a. AS, B-4901, HLAB)
  • Promoter none
  • Tag / Fusion Protein
    • his (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer CAG GAA ACA GCT ATG ACC
  • 3′ sequencing primer TGT AAA ACG ACG GCC AGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-HLA-B0702-His was a gift from Sébastien Walchli (Addgene plasmid # 104849 ; http://n2t.net/addgene:104849 ; RRID:Addgene_104849)

  • For your References section:

    Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8(+) T Cell Responses against Nonstructural Viral Proteins in Acute Human Tick-Borne Encephalitis Virus Infection. Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J, Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J, Schumacher TN, Ljunggren HG, Sandberg JK, Achour A, Gredmark-Russ S. Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 10.4049/immunohorizons.1800029. 10.4049/immunohorizons.1800029 PubMed 31022685
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