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pMP71-HLA-B15-His
(Plasmid #104848)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104848 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMP71
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6750
  • Modifications to backbone
    converted to become Gateway compatible, see this paper: PMID: 22132171
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    major histocompatibility complex, class I, B
  • Alt name
    HLA-B150101
  • Species
    H. sapiens (human)
  • Mutation
    2 differences compared to original B15: L2->R and M4-> T, no change was observed on final product.
  • Entrez Gene
    HLA-B (a.k.a. AS, B-4901, HLAB)
  • Promoter LTR
  • Tag / Fusion Protein
    • his (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP71-HLA-B15-His was a gift from Sébastien Walchli (Addgene plasmid # 104848 ; http://n2t.net/addgene:104848 ; RRID:Addgene_104848)
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