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Purposeb2AR-SPARK protease component
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX208
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-arrestin2-HA-TEV219
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX208-beta-arrestin2-HA-TEV219 was a gift from Alice Ting (Addgene plasmid # 104846 ; http://n2t.net/addgene:104846 ; RRID:Addgene_104846) -
For your References section:
Time-gated detection of protein-protein interactions with transcriptional readout. Kim MW, Wang W, Sanchez MI, Coukos R, von Zastrow M, Ting AY. Elife. 2017 Nov 30;6. doi: 10.7554/eLife.30233. 10.7554/eLife.30233 PubMed 29189201