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PurposeeFFly luciferase for invivo Imaging, eGFP for flourescent detection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104834 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH-EF1a-MCS-T2A-copGFP
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Backbone manufacturerSystem Biosciences
- Backbone size w/o insert (bp) 6481
- Total vector size (bp) 8842
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Vector typeMammalian Expression, Lentiviral, Luciferase ; eGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameeFFly
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Insert Size (bp)1641
Gene/Insert 2
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Gene/Insert nameeGFP
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-EF1a-eFFly-eGFP was a gift from Irmela Jeremias (Addgene plasmid # 104834 ; http://n2t.net/addgene:104834 ; RRID:Addgene_104834) -
For your References section:
Dual PI3K/mTOR inhibition shows antileukemic activity in MLL-rearranged acute myeloid leukemia. Sandhofer N, Metzeler KH, Rothenberg M, Herold T, Tiedt S, Groiss V, Carlet M, Walter G, Hinrichsen T, Wachter O, Grunert M, Schneider S, Subklewe M, Dufour A, Frohling S, Klein HG, Hiddemann W, Jeremias I, Spiekermann K. Leukemia. 2015 Apr;29(4):828-38. doi: 10.1038/leu.2014.305. Epub 2014 Oct 17. 10.1038/leu.2014.305 PubMed 25322685