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pENTR-li-p33_RS-CLIP
(Plasmid #104634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104634 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 3510
  • Vector type
    entry vector for Gateway system

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    modified Invariant chain p33
  • Alt name
    Ii
  • Alt name
    CD74
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    602
  • Mutation
    CLIP domain was replaced by 2 restriction sites
  • GenBank ID
    CD74 molecule (Homo sapiens) NM_001025159.2
  • Entrez Gene
    CD74 (a.k.a. CLIP, DHLAG, HLADG, II, Ia-GAMMA, p33)
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer CAG GAA ACA GCT ATG ACC
  • 3′ sequencing primer TGT AAA ACG ACG GCC AGT
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Tone F. Gregers, received through a collaboration

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The original gene comes from Dr. Tone Gregers previous construct, we subcloned it into pENTR and performed the modification of the CLIP domain

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-li-p33_RS-CLIP was a gift from Sébastien Walchli (Addgene plasmid # 104634 ; http://n2t.net/addgene:104634 ; RRID:Addgene_104634)

  • For your References section:

    Antigen-delivery through invariant chain (CD74) boosts CD8 and CD4 T cell immunity. Mensali N, Grenov A, Pati NB, Dillard P, Myhre MR, Gaudernack G, Kvalheim G, Inderberg EM, Bakke O, Walchli S. Oncoimmunology. 2019 Jan 11;8(3):1558663. doi: 10.1080/2162402X.2018.1558663. eCollection 2019. 10.1080/2162402X.2018.1558663 PubMed 30723591
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