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Addgene

pSBY3_ligD-HygR
(Plasmid #104625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMV261
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FnCpf1
  • Mutation
    FnCpf1 codon-optimized C.glutamicum
  • Promoter hsp60

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The other inserted are "crRNA to ligD" and "hygromysin resistance gene". "crRNA to ligD" species is synthetic, promoter is Pj23119. Shuttle vector Mycobacterium smegmatis/E.coli

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBY3_ligD-HygR was a gift from Sheng Yang (Addgene plasmid # 104625 ; http://n2t.net/addgene:104625 ; RRID:Addgene_104625)

  • For your References section:

    A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. Sun B, Yang J, Yang S, Ye RD, Chen D, Jiang Y. Biotechnol J. 2018 Sep;13(9):e1700588. doi: 10.1002/biot.201700588. Epub 2018 Aug 6. 10.1002/biot.201700588 PubMed 30039929
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