pJC-20xUAS-TALE-VP64-P10
(Plasmid
#104605)
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Purpose(Empty Backbone) Control plasmid (no TALE array) with P10 terminator
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJC-TALE-VP64 (Plasmid #46147)
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Backbone manufacturerDavid L. Stern
- Backbone size (bp) 10365
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Modifications to backboneSV40 terminator replaced with P10 terminator
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Vector typeInsect Expression, TALEN
- Promoter hsp70
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byP10 terminator was amplified from plasmid pJFRC81 (Plasmid #36432)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC-20xUAS-TALE-VP64-P10 was a gift from Tudor Fulga (Addgene plasmid # 104605 ; http://n2t.net/addgene:104605 ; RRID:Addgene_104605) -
For your References section:
A multiplexable TALE-based binary expression system for in vivo cellular interaction studies. Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA. Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3. 10.1038/s41467-017-01592-3 PubMed 29162808