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pCA528 CHMP3 159-222
(Plasmid #104599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCA528
  • Backbone size w/o insert (bp) 5597
  • Total vector size (bp) 5793
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHMP3 residues 159-222
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    201
  • Mutation
    Contains nonnative Gly-Cys for fluorescent labeling
  • Entrez Gene
    CHMP3 (a.k.a. CGI-149, NEDF, VPS24)
  • Promoter T7
  • Tag / Fusion Protein
    • His-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCA528 CHMP3 159-222 was a gift from Wesley Sundquist (Addgene plasmid # 104599 ; http://n2t.net/addgene:104599 ; RRID:Addgene_104599)

  • For your References section:

    Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI. Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779. 10.7554/eLife.77779 PubMed 36107470
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