pCA528 CHMP1A 140-196
(Plasmid #104593)

• Purpose: Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1
• Depositing Lab: Wesley Sundquist
• Publication: Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779.

Backbone

• Vector backbone: pCA528
• Backbone size w/o insert (bp): 5597
• Total vector size (bp): 5772
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHMP1A residues 140-196
• Species: H. sapiens (human)
• Insert Size (bp): 174
• Mutation: Contains nonnative Gly-Cys for fluorescent labeling
• Entrez Gene: CHMP1A (a.k.a. CHMP1, PCH8, PCOLN3, PRSM1, VPS46-1, VPS46A)
• Promoter: T7
• Tag / Fusion Protein:
  • His-SUMO (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 term

Resource Information

• Supplemental Documents:
  • pCA528CHMP1A140-196.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.02.09.477148v1