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Addgene

pG-HIV-SIN-2LTR
(Plasmid #104591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGemT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3001
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2LTR circle junction of self-inactivating HIV-based vectors
  • Species
    HIV-1 based vector

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer T7-F TAATACGACTCACTATAGGG and SP6-F ATTTAGGTGACACTATAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The self-inactivating (SIN) HIV-1 vector has sequence from the LTR deleted, including the sequence encoded by qPCR primer MH536. An alternative primer set and qPCR standard plasmid was made to detect HIV-1 SIN vector 2LTR circles. The 2LTR circle insert is Addgene 753-929 bp. It may be amplified with primers MH535 (5' AACTAGGGAACCCACTGCTTAAG), KY268 (5' CCAGAGAGACCCAGTACAAGCA), and Taqman probe MH603 (5' ACACTACTTGAAGCACTCAAGGCAAGCTTT)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG-HIV-SIN-2LTR was a gift from Kristine Yoder (Addgene plasmid # 104591 ; http://n2t.net/addgene:104591 ; RRID:Addgene_104591)

  • For your References section:

    Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles. Yoder KE, Fishel R. J Virol Methods. 2008 Nov;153(2):253-6. doi: 10.1016/j.jviromet.2008.07.032. Epub 2008 Sep 17. 10.1016/j.jviromet.2008.07.032 PubMed 18762215
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