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Addgene

pAAV-EFS-SpCas9
(Plasmid #104588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX551
  • Backbone manufacturer
    Addgene #60957
  • Backbone size w/o insert (bp) 2905
  • Total vector size (bp) 7429
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptococcus pyogenes Cas9
  • Alt name
    SpCas9
  • Species
    Synthetic
  • Insert Size (bp)
    4200
  • Promoter EFS
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer GCCTCTAGAAAGCTTAGGTCTTGAAAGGAGTGG
  • 3′ sequencing primer ATGGAACAAAAACTCATCTCAGAAGAGGATCTGTCGCCGAAGAAAAAGCGCAAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EFS-SpCas9 was a gift from Ryohei Yasuda (Addgene plasmid # 104588 ; http://n2t.net/addgene:104588 ; RRID:Addgene_104588)

  • For your References section:

    Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain. Nishiyama J, Mikuni T, Yasuda R. Neuron. 2017 Oct 18. pii: S0896-6273(17)30933-9. doi: 10.1016/j.neuron.2017.10.004. 10.1016/j.neuron.2017.10.004 PubMed 29056297
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