pHIV-iRFP720-E2A-Luc
(Plasmid #104587)

• Purpose: Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter
• Depositing Lab: Antonius Plagge
• Publication: Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 10.7554/eLife.33140.

Backbone

• Vector backbone: pHIV-Luciferase
• Vector type: Mammalian Expression, Lentiviral, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: iRFP720-E2A-Luciferase
• Species: firefly
• Insert Size (bp): 2661
• Tag / Fusion Protein:
  • bi-cistronic (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We constructed this vector from precursor plasmids pHIV-Luciferase (Addgene #21375) and piRFP720-N1 (Addgene #45461)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The vector leads to expression of iRFP720 and luciferase as separate proteins for fluorescence, optoacoustic and bioluminescence imaging.

The full pHIV-iRFP720-E2A-Luc sequence is due to be released by Genbank on 13th Nov. 2017 with accession number MF693179

bioRxiv preprint first posted online Oct. 19, 2017; doi: http://dx.doi.org/10.1101/199836