-5.5kb-UCP1-GFP
(Plasmid #104585)

• Purpose: Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression
• Depositing Lab: Irfan Lodhi
• Publication: Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774. doi: 10.1016/j.celrep.2017.08.077.

Backbone

• Vector backbone: pCMV6-AC-GFP
• Backbone manufacturer: Origene
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UCP1 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 5500
• Entrez Gene: Ucp1 (a.k.a. Slc25a7, Ucp)
• Promoter: UCP1
• Tag / Fusion Protein:
  • TurboGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CMV promoter driving GFP expression was removed and replaced with the -5.5 kb mouse UCP1 promoter + Kozak

Reference sequence for promoter may be found here:
http://epd.vital-it.ch/cgi-bin/get_doc?db=mmEpdNew&format=genome&entry=Ucp1_1