pTrig-CopA
(Plasmid
#104578)
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PurposeCopy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104578 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemini-F
- Total vector size (bp) 4248
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePCopA-RiboJ-B0034-repL
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrig-CopA was a gift from Harris Wang (Addgene plasmid # 104578 ; http://n2t.net/addgene:104578 ; RRID:Addgene_104578) -
For your References section:
Multiplex recording of cellular events over time on CRISPR biological tape. Sheth RU, Yim SS, Wu FL, Wang HH. Science. 2017 Dec 15;358(6369):1457-1461. doi: 10.1126/science.aao0958. Epub 2017 Nov 23. 10.1126/science.aao0958 PubMed 29170279
