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JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)
(Plasmid #104570)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104570 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-GFP
  • Backbone manufacturer
    Cepko lab (Addgene plasmid # 11179)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dLbCpf1(D832A)-DmrA(x3)
  • Alt name
    human codon optimized ‘dead’ Cpf1 fused to three DmrA domains
  • Species
    Lachnospiraceae bacterium
  • Mutation
    D832A
  • Promoter CAG
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x HA (C terminal on insert)
    • 3x FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer oBK23-R (5'-CAGAGGGAAAAAGATCTCAGTGG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3) was a gift from Keith Joung (Addgene plasmid # 104570 ; http://n2t.net/addgene:104570 ; RRID:Addgene_104570)

  • For your References section:

    Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman JS, Joung JK. Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483. 10.1038/nmeth.4483 PubMed 29083402
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