JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)
(Plasmid #104568)

• Purpose: Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain
• Depositing Lab: Keith Joung
• Publication: Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.

Backbone

• Vector backbone: pCAG-GFP
• Backbone manufacturer: Cepko lab (Addgene plasmid # 11179)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dLbCpf1(D832A)-DmrA(x1)
• Alt name: human codon optimized ‘dead’ Cpf1 fused to one DmrA domain
• Species: Lachnospiraceae bacterium
• Mutation: D832A
• Promoter: CAG
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • 3x HA (C terminal on insert)
  • 3x FLAG (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: oBK23-R (5'-CAGAGGGAAAAAGATCTCAGTGG-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry