JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65
(Plasmid
#104566)
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PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG-GFP
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Backbone manufacturerCepko lab (Addgene plasmid # 11179)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedLbCpf1(D832A)-p65
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Alt namehuman codon optimized ‘dead’ Cpf1 fused to NF-KB p65 activation domain
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SpeciesLachnospiraceae bacterium
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MutationD832A
- Promoter CAG
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Tags
/ Fusion Proteins
- NLS (C terminal on insert)
- 3x HA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
- 3′ sequencing primer oBK23-R (5'-CAGAGGGAAAAAGATCTCAGTGG-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65 was a gift from Keith Joung (Addgene plasmid # 104566 ; http://n2t.net/addgene:104566 ; RRID:Addgene_104566) -
For your References section:
Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman JS, Joung JK. Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483. 10.1038/nmeth.4483 PubMed 29083402