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Addgene

PB-U6insert-EF1puro
(Plasmid #104537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB
  • Backbone manufacturer
    Systems Biosciences
  • Backbone size (bp) 6300
  • Vector type
    Mammalian Expression, Mouse Targeting, CRISPR, Synthetic Biology ; Piggybac transposon
  • Promoter U6
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-U6insert-EF1puro was a gift from George Church (Addgene plasmid # 104537 ; http://n2t.net/addgene:104537 ; RRID:Addgene_104537)

  • For your References section:

    Developmental barcoding of whole mouse via homing CRISPR. Kalhor R, Kalhor K, Mejia L, Leeper K, Graveline A, Mali P, Church GM. Science. 2018 Aug 9. pii: science.aat9804. doi: 10.1126/science.aat9804. 10.1126/science.aat9804 PubMed 30093604
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