pILGFP4I
(Plasmid
#104504)
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PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104504 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSkGAL1 promoter-yEGFP
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SpeciesSaccharomyces kudriavzevii
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pILGFP4I was a gift from Claudia Vickers (Addgene plasmid # 104504 ; http://n2t.net/addgene:104504 ; RRID:Addgene_104504) -
For your References section:
An Expanded Heterologous GAL Promoter Collection for Diauxie-Inducible Expression in Saccharomyces cerevisiae. Peng B, Wood RJ, Nielsen LK, Vickers CE. ACS Synth Biol. 2018 Feb 16;7(2):748-751. doi: 10.1021/acssynbio.7b00355. Epub 2018 Jan 23. 10.1021/acssynbio.7b00355 PubMed 29301066