TDP-43_NTD1-80_E17R
(Plasmid
#104479)
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Purposebacterial expression of TDP-43 NTD E17R
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ411
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTDP-43
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SpeciesH. sapiens (human)
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MutationE17R
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TDP-43_NTD1-80_E17R was a gift from Nicolas Fawzi (Addgene plasmid # 104479 ; http://n2t.net/addgene:104479 ; RRID:Addgene_104479) -
For your References section:
A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing. Wang A, Conicella AE, Schmidt HB, Martin EW, Rhoads SN, Reeb AN, Nourse A, Ramirez Montero D, Ryan VH, Rohatgi R, Shewmaker F, Naik MT, Mittag T, Ayala YM, Fawzi NL. EMBO J. 2018 Mar 1;37(5). pii: embj.201797452. doi: 10.15252/embj.201797452. Epub 2018 Feb 9. 10.15252/embj.201797452 PubMed 29438978