pBluescriptKS(-)-FRB
(Plasmid #104475)

• Purpose: Plasmid carrying FRB with C-terminal HA tag.
• Depositing Lab: Benjamin Glick
• Publication: Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi: 10.7554/eLife.13232.

Backbone

• Vector backbone: pBlueScript KS (-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2958
• Total vector size (bp): 3317
• Vector type: phagemid

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FRB
• Alt name: FKBP-rapamycin binding domain
• Species: H. sapiens (human)
• Insert Size (bp): 378
• Mutation: includes only the FRB domain
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13 REVERSE
• 3′ sequencing primer: M13 FORWARD

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The FRB gene was obtained from Ariad Pharmaceuticals (Cambridge, MA).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry