YIplac211-RPL13A-FKBPx4
(Plasmid #104474)

• Purpose: Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.
• Depositing Lab: Benjamin Glick
• Publication: Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi: 10.7554/eLife.13232.

Backbone

• Vector backbone: YIplac211
• Backbone size w/o insert (bp): 3797
• Total vector size (bp): 6463
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPL13Ap
• Alt name: YDL082W
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1334
• Mutation: includes the last 354 bp of RPL13A only
• Entrez Gene: RPL13A (a.k.a. YDL082W)
• Tag / Fusion Protein:
  • FKBPx4-HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13 REVERSE
• 3′ sequencing primer: M13 FORWARD

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The FKBP gene was obtained from Ariad Pharmaceuticals (Cambridge, MA).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry