YIplac204-TC-OM45-FKBPx4
(Plasmid #104472)

• Purpose: Integrating plasmid for overexpression of OM45-FKBPx4.
• Depositing Lab: Benjamin Glick
• Publication: Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi: 10.7554/eLife.13232.

Backbone

• Vector backbone: YIplac204
• Backbone size w/o insert (bp): 3545
• Total vector size (bp): 6649
• Modifications to backbone: TPI1 promoter, CYC1 terminator
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OM45p
• Alt name: YIL136W
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1178
• Entrez Gene: OM45 (a.k.a. YIL136W)
• Promoter: TPI1
• Tag / Fusion Protein:
  • FKBPx4- HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: BO17
• 3′ sequencing primer: BO18

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The FKBP gene was obtained from Ariad Pharmaceuticals (Cambridge, MA).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry