hnRNPA2_LC_P298L_S285C
(Plasmid
#104469)
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Purposeexpress His tagged P298L S285C hnRNPA2 LC
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104469 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ411
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehnRNPA2
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SpeciesH. sapiens (human)
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MutationP298L S285C
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Entrez GeneHNRNPA2B1 (a.k.a. HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, OPMD2, RNPA2, SNRPB1)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hnRNPA2_LC_P298L_S285C was a gift from Nicolas Fawzi (Addgene plasmid # 104469 ; http://n2t.net/addgene:104469 ; RRID:Addgene_104469) -
For your References section:
Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation. Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, Amaya J, Burke KA, Mittal J, Fawzi NL. Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 10.1016/j.molcel.2017.12.022. 10.1016/j.molcel.2017.12.022 PubMed 29358076