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Addgene

PB-TRE-EGFP-EF1a-rtTA
(Plasmid #104454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104454 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone manufacturer
    The piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800
  • Total vector size (bp) 8316
  • Vector type
    Mammalian Expression ; PiggyBac
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    enhanced green fluorescent protein
  • Alt name
    EGFP
  • Insert Size (bp)
    717

Gene/Insert 2

  • Gene/Insert name
    reverse tetracycline-controlled transactivator 3
  • Alt name
    rtTA3
  • Insert Size (bp)
    744

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TRE-EGFP-EF1a-rtTA was a gift from Volker Busskamp (Addgene plasmid # 104454 ; http://n2t.net/addgene:104454 ; RRID:Addgene_104454)

  • For your References section:

    Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders. Sauter EJ, Kutsche LK, Klapper SD, Busskamp V. Methods Mol Biol. 2019;1942:101-121. doi: 10.1007/978-1-4939-9080-1_9. 10.1007/978-1-4939-9080-1_9 PubMed 30900179
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