GST(LSCS)GFP-ActA
(Plasmid
#104452)
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PurposeMitophagy cargo assay
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4754
- Total vector size (bp) 6095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGST-GFP-ActA
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SpeciesGFP is from Aequorea victoria
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Insert Size (bp)1470
- Promoter CMV
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nru1 (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer SP6 5'GATTTAGGTGACACTATAG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST(LSCS)GFP-ActA was a gift from Carol Mercer (Addgene plasmid # 104452 ; http://n2t.net/addgene:104452 ; RRID:Addgene_104452) -
For your References section:
The GST-BHMT assay and related assays for autophagy. Dennis PB, Mercer CA. Methods Enzymol. 2009;452:97-118. doi: 10.1016/S0076-6879(08)03607-0. 10.1016/S0076-6879(08)03607-0 PubMed 19200878
Map uploaded by the depositor.
