Clim Actin MCH Puro (CAMP)
(Plasmid
#104446)
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PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1
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Backbone manufacturerThermoFisher
- Backbone size w/o insert (bp) 3800
- Total vector size (bp) 4510
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Vector typeExpression in Corallochytrium (single-celled eukaryote)
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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Insert Size (bp)700
- Promoter actin
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13R CAGGAAACAGCTATGAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.11.12.379420v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Clim Actin MCH Puro (CAMP) was a gift from Elena Casacuberta & Inaki Ruiz-Trillo (Addgene plasmid # 104446 ; http://n2t.net/addgene:104446 ; RRID:Addgene_104446) -
For your References section:
Stable transfection in protist Corallochytrium limacisporum identifies novel cellular features among unicellular animals relatives. Kozyczkowska A, Najle SR, Ocana-Pallares E, Areste C, Shabardina V, Ara PS, Ruiz-Trillo I, Casacuberta E. Curr Biol. 2021 Sep 27;31(18):4104-4110.e5. doi: 10.1016/j.cub.2021.06.061. Epub 2021 Jul 21. 10.1016/j.cub.2021.06.061 PubMed 34293333
Map uploaded by the depositor.
