GST-BHMT-I/G
(Plasmid #104442)

• Purpose: Autophagy cargo assay
• Depositing Lab: Carol Mercer
• Publication: Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 2007 Nov 7.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4754
• Total vector size (bp): 7832
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BHMT
• Species: H. sapiens (human)
• Insert Size (bp): 3213
• Mutation: N43A in GST (in a potential KFERQ-like sequence). BHMT does not contain the first 8 or last 9 amino acids.
• Entrez Gene: BHMT (a.k.a. BHMT1, HEL-S-61p)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GST (N terminal on insert)
  • GFP-myc GFP is from Aequorea victoria (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nru1 (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: SP6 5'-TGATGAGTTTGGACAAACCAC-3'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GFP is from Aequorea victoria