sg resistant gamma-tubulin
(Plasmid #104433)

• Purpose: For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA
• Depositing Lab: Maria Alvarado-Kristensson
• Publication: Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii: S0167-4889(17)30283-5. doi: 10.1016/j.bbamcr.2017.10.008.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6785
• Modifications to backbone: NA
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gamma-tubulin 1
• Alt name: TUBG1
• Species: H. sapiens (human)
• Insert Size (bp): 1356
• Mutation: changed t bp28 to c; g bp30 to a; c bp33 to a; and g bp36 to a. These are silenced mutations.
• GenBank ID:
• Entrez Gene: TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: BGH Reverse Primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TUBG1 was originally cloned by Dr. Jiri Bartek.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry