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Addgene

pKL07
(Plasmid #104431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p416
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 8015
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mito-Super Ecliptic pHluorin-mRuby2
  • Alt name
    SU9-SEP-Cterminal-mRuby2
  • Species
    Synthetic
  • Insert Size (bp)
    1572
  • Promoter Tef1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTCTTTCGATGACCTCCCATTG
  • 3′ sequencing primer CTTCAGGTTGTCTAACTCCTTCC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ordered as gBlock from IDT

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKL07 was a gift from Joel Kralj (Addgene plasmid # 104431 ; http://n2t.net/addgene:104431 ; RRID:Addgene_104431)

  • For your References section:

    Live Cell Imaging Reveals pH Oscillations in Saccharomyces cerevisiae During Metabolic Transitions. Dodd BJT, Kralj JM. Sci Rep. 2017 Oct 24;7(1):13922. doi: 10.1038/s41598-017-14382-0. 10.1038/s41598-017-14382-0 [pii] PubMed 29066766
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