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Addgene

p-dCas9-HDAC1-H141A-Hygro
(Plasmid #104417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMLM3705
  • Backbone manufacturer
    Keith Joung (Addgene plasmid # 47754)
  • Backbone size w/o insert (bp) 9075
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dHDAC1
  • Species
    H. sapiens (human)
  • Mutation
    H141A
  • Entrez Gene
    HDAC1 (a.k.a. GON-10, HD1, KDAC1, RPD3, RPD3L1)
  • Promoter CMV promoter

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). HDAC1 (a.a. 4-384) was from Addgene plasmid 13820 (HDAC1 Flag). The H141A mutation was added by PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-dCas9-HDAC1-H141A-Hygro was a gift from Stephan Beck (Addgene plasmid # 104417 ; http://n2t.net/addgene:104417 ; RRID:Addgene_104417)
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