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pLenti6.3/TO/CD9-GFP
(Plasmid #104402)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.3/TO/V5-DEST
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 9351
  • Total vector size (bp) 9227
  • Modifications to backbone
    Gateway inserted
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD9
  • Alt name
    BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1431
  • GenBank ID
    NM_001769
  • Entrez Gene
    CD9 (a.k.a. BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid addgene #54029
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is used and named CD9-GFP in publication even though it contains mEmerald. Described in the methods of Boker et al 2018 (https://www.ncbi.nlm.nih.gov/pubmed/29221804) Please see publication for more information.

Also see the following article for additional information on using this plasmid: Schiller et al., 2018 https://www.sciencedirect.com/science/article/pii/S2329050118300378

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3/TO/CD9-GFP was a gift from Jens Gruber (Addgene plasmid # 104402 ; http://n2t.net/addgene:104402 ; RRID:Addgene_104402)

  • For your References section:

    The Impact of the CD9 Tetraspanin on Lentivirus Infectivity and Exosome Secretion. Boker KO, Lemus-Diaz N, Rinaldi Ferreira R, Schiller L, Schneider S, Gruber J. Mol Ther. 2018 Feb 7;26(2):634-647. doi: 10.1016/j.ymthe.2017.11.008. Epub 2017 Nov 16. 10.1016/j.ymthe.2017.11.008 PubMed 29221804
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