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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
(Plasmid #104394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YcpLac111
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCP-NLS-2xyeGFP
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    1827
  • Mutation
    MCP fused to NLS(SV40) and 2x yeast codon optimized eGFP
  • Promoter CYC1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuII (unknown if destroyed)
  • 3′ cloning site PvuII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert expressed under the control of the CYC1 promoter and terminator

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP was a gift from Robert Singer & Evelina Tutucci (Addgene plasmid # 104394 ; http://n2t.net/addgene:104394 ; RRID:Addgene_104394)

  • For your References section:

    An improved MS2 system for accurate reporting of the mRNA life cycle. Tutucci E, Vera M, Biswas J, Garcia J, Parker R, Singer RH. Nat Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502. 10.1038/nmeth.4502 PubMed 29131164
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