pET251-pUC 12xMS2V6 Loxp KANr Loxp
(Plasmid #104392)

• Purpose: Yeast endogenous mRNA tagging
• Depositing Labs: Robert Singer, Evelina Tutucci
• Publication: Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502.

Backbone

• Vector backbone: pUC
• Vector type: Bacterial Expression
• Selectable markers: Geneticin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 12xMS2V6
• Species: Synthetic
• Insert Size (bp): 828
• Mutation: All Loops are U-variants, interspaced by 50 nucleotide linkers, have synonymous sequence,and the sequence is ORF in the 3 frames

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: SalI (destroyed during cloning)

Resource Information

• Supplemental Documents:
  • pET251-pUC 12xMS2V6 Loxp KANr Loxp
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert cloned from pET246 as BamHI SalI in Addgene plasmid #45162, replacing previous MS2 variant