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pET259-pUC57 24xMS2V6
(Plasmid #104391)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genescript
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    24xMS2V6
  • Species
    Synthetic
  • Insert Size (bp)
    1662
  • Mutation
    All Loops are U-variants, interspaced by 50 nucleotide linkers, have synonymous sequence,and the sequence is ORF in the 3 frames

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BglII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert generated by duplication of 12xMBSV6 as a BamHI/BglII fragment inserted at the BglII site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET259-pUC57 24xMS2V6 was a gift from Robert Singer & Evelina Tutucci (Addgene plasmid # 104391 ; http://n2t.net/addgene:104391 ; RRID:Addgene_104391)

  • For your References section:

    An improved MS2 system for accurate reporting of the mRNA life cycle. Tutucci E, Vera M, Biswas J, Garcia J, Parker R, Singer RH. Nat Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502. 10.1038/nmeth.4502 PubMed 29131164
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