PB-eDIO-EYFP
(Plasmid
#104388)
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PurposePiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggyBac
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Backbone manufacturerThe piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800
- Backbone size w/o insert (bp) 8857
- Total vector size (bp) 9601
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Vector typeMammalian Expression, Cre/Lox ; PiggyBac
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnhanced Yellow Fluorescent Protein
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Alt nameEYFP
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SpeciesSynthetic
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Insert Size (bp)744
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byKarl Deisseroth, Addgene Plasmid #35507
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-eDIO-EYFP was a gift from Volker Busskamp (Addgene plasmid # 104388 ; http://n2t.net/addgene:104388 ; RRID:Addgene_104388) -
For your References section:
On-demand optogenetic activation of human stem-cell-derived neurons. Klapper SD, Sauter EJ, Swiersy A, Hyman MAE, Bamann C, Bamberg E, Busskamp V. Sci Rep. 2017 Oct 31;7(1):14450. doi: 10.1038/s41598-017-14827-6. 10.1038/s41598-017-14827-6 [pii] PubMed 29089561