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Addgene

PB-eDIO-EYFP
(Plasmid #104388)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104388 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PiggyBac
  • Backbone manufacturer
    The piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800
  • Backbone size w/o insert (bp) 8857
  • Total vector size (bp) 9601
  • Vector type
    Mammalian Expression, Cre/Lox ; PiggyBac
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced Yellow Fluorescent Protein
  • Alt name
    EYFP
  • Species
    Synthetic
  • Insert Size (bp)
    744

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Karl Deisseroth, Addgene Plasmid #35507

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-eDIO-EYFP was a gift from Volker Busskamp (Addgene plasmid # 104388 ; http://n2t.net/addgene:104388 ; RRID:Addgene_104388)
  • For your References section:

    On-demand optogenetic activation of human stem-cell-derived neurons. Klapper SD, Sauter EJ, Swiersy A, Hyman MAE, Bamann C, Bamberg E, Busskamp V. Sci Rep. 2017 Oct 31;7(1):14450. doi: 10.1038/s41598-017-14827-6. 10.1038/s41598-017-14827-6 [pii] PubMed 29089561