PB-eDIO-ChR2-EYFP
(Plasmid #104387)

• Purpose: PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
• Depositing Lab: Volker Busskamp
• Publication: Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10.1038/s41598-017-14827-6.

Backbone

• Vector backbone: PiggyBac
• Backbone manufacturer: The piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800
• Backbone size w/o insert (bp): 8860
• Total vector size (bp): 10522
• Vector type: Mammalian Expression, Cre/Lox ; PiggyBac
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Channelrhodopsin 2
• Alt name: hChR2
• Species: Chlamydomonas reinhardtii
• Insert Size (bp): 1662
• Mutation: E134R
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • PB-eDIO-ChR2-EYFP_Snap.gb
• A portion of this plasmid was derived from a plasmid made by: Karl Deisseroth, Addgene Plasmid #35507

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry