pCDNA-hNgAgo
(Plasmid #104384)

• Purpose: human codon optimized NgAgo; pCDNA3 vector backbone, mammalian expression
• Depositing Lab: David Segal
• Publication: O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. doi: 10.1371/journal.pone.0193818. eCollection 2018.

Backbone

• Vector backbone: pcDNA3.3-TOPO
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 5407
• Total vector size (bp): 8476
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hNgAgo
• Alt name: Protein Sequence ID: WP_005580376
• Alt name: Gene: Natgr_2597
• Alt name: Stem cell self-renewal protein Piwi domain protein
• Species: Synthetic; human codon-optimized Agonaute from Natronobacterium gregoryi
• Insert Size (bp): 2661
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3XFlag (N terminal on insert)
  • SV40 NLS (N terminal on insert)
  • Nucleoplasmin NLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: polyA TK-R

Resource Information

• Supplemental Documents:
  • pcDNA-hNgAgo.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry