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Addgene

pCDNA-hTtAgo
(Plasmid #104383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.3-TOPO
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 5407
  • Total vector size (bp) 7870
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hTtAgo
  • Alt name
    Protein Sequence ID: WP_011174533
  • Alt name
    Gene: TT_P0026
  • Species
    Synthetic; human codon-optimized Agonaute from Thermus thermophilus
  • Insert Size (bp)
    2055
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3XFlag (N terminal on insert)
    • SV40 NLS (N terminal on insert)
    • Nucleoplasmin NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA-hTtAgo was a gift from David Segal (Addgene plasmid # 104383 ; http://n2t.net/addgene:104383 ; RRID:Addgene_104383)

  • For your References section:

    Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ. PLoS One. 2018 Mar 27;13(3):e0193818. doi: 10.1371/journal.pone.0193818. eCollection 2018. 10.1371/journal.pone.0193818 PubMed 29584750
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