SLC30A10-GFP
(Plasmid #104381)

• Purpose: Expresses SLC30A10-GFP wild-type in mammalian cells
• Depositing Lab: Somshuvra Mukhopadhyay
• Publication: Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-3262. doi: 10.1083/jcb.201704015. Epub 2017 Sep 7.

Backbone

• Vector backbone: pEGFP-N3
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC30A10
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Entrez Gene: SLC30A10 (a.k.a. HMDPC, HMNDYT1, ZNT10, ZNT8, ZRC1, ZnT-10)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry