SLC39A14-GFP
(Plasmid #104380)

• Purpose: Expresses SLC39A14/ZIP14 wild-type in mammalian cells
• Depositing Lab: Somshuvra Mukhopadhyay
• Publication: Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16615. doi: 10.1074/jbc.M117.804989. Epub 2017 Aug 31.

Backbone

• Vector backbone: pEGFP-N3
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SLC39A14
• Alt name: ZIP14
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Entrez Gene: SLC39A14 (a.k.a. HMNDYT2, LZT-Hs4, NET34, ZIP14, cig19)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GTC GGT ACC ATG AAG CTG CTG CTG CTG CAC
• 3′ sequencing primer: CGCGGATCCCCCAATCTGGATCTGTCCTGAATA

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry